Medina Spirit was the first horse to cross the finish line during the race, but after having a positive drug test, Mandaloun has been declared the race winner, The Associated Press reported.
Mandaloun had finished in second place, WLKY reported.
Medina Spirit died on Dec. 6 after a workout at Santa Anita. A necropsy was performed earlier this month and at the time showed no definitive cause of death, the AP reported.
The horse had failed a drug test shortly after the Kentucky Derby, WLKY reported. He had 21 picograms of betamethasone, about twice the legal limit of the steroid. It is legal in Kentucky except on race day.
In addition to Medina Spirit losing the title, his trainer, Bob Baffert, has been suspended for 90 days and will have to pay a fine of $7,500, WLKY reported.
Baffert said the steroids were from an ointment he used on a skin condition and was not injected into the horse.
Baffert was already suspended by Churchill Downs and was not allowed to enter a horse in the 2022 and 2023 Kentucky Derbies, WLKY reported. He also lost his record-breaking seventh Kentucky Derby victory, The New York Times reported.
He’s also not allowed to enter horses at tracks in Belmont, Saratoga and Aqueduct.
Medina Spirit’s owner Amr Zedan will not get the $1.8 million check from the Derby win. It will now go to Mandaloun’s owner, Juddmonte, which was founded by Saudi Arabian Prince Khalid bin Abdullah, the Times reported.
2021 Kentucky Derby Horses work out as the sun rises at Churchill Downs Friday, April 30, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A women watches a race before the 147th running of the Kentucky Oaks at Churchill Downs, Friday, April 30, 2021, in Louisville, Ky.(AP Photo/Michael Conroy) (Michael Conroy/AP)
2021 Kentucky Derby A lady wears a stylish hat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Mark Ferguson wears a coronavirus-themed hat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Kentucky Derby entrant Essential Quality works out at Churchill Downs Thursday, April 29, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Kentucky Derby entrant Mandaloun works out at Churchill Downs Thursday, April 29, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Kentucky Derby entrant Rock Your World works out at Churchill Downs Thursday, April 29, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Kentucky Derby entrant Hot Rod Charlie works out at Churchill Downs Thursday, April 29, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A horse works out at Churchill Downs Friday, April 30, 2021, in Louisville, Ky. The 147th running of the Kentucky Derby is scheduled for Saturday, May 1. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A couple looks over a racing program before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Fans watch race six before 147th running of the Kentucky Oaks at Churchill Downs, Friday, April 30, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Umberto Rispoli rides Visitant to the lead in race six before 147th running of the Kentucky Oaks at Churchill Downs, Friday, April 30, 2021, in Louisville, Ky. (AP Photo/Michael Conroy) (Michael Conroy/AP)
2021 Kentucky Derby A man looks over a racing program before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A man wears Kentucky Derby-themed socks before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Fans wait for a race while social distancing before 147th running of the Kentucky Oaks at Churchill Downs, Friday, April 30, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Sara Edwards watches a race before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Fans walk to the infield for 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Soldiers walk through a tunnel before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A man places a bet before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman takes a photo before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A man places a bet before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby Women walk to their seats before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman takes a photo before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Michael Conroy) (Michael Conroy/AP)
2021 Kentucky Derby A fan watches a race from the infield before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Roses bloom during race three before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Darron Cummings) (Darron Cummings/AP)
2021 Kentucky Derby A women wears a hat and a horse racing face mask before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Women walk to their seats before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman watches a race before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman walks to her seat before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby Fans watch as horses run a race before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A woman watches a race before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Michael Conroy) (Michael Conroy/AP)
2021 Kentucky Derby Men talk as they watch a race before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Michael Conroy) (Michael Conroy/AP)
2021 Kentucky Derby A woman adjust her face mask before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)
2021 Kentucky Derby A man looks at a racing program before 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Charlie Riedel) (Charlie Riedel/AP)
2021 Kentucky Derby A man looks at a racing program before the 147th running of the Kentucky Derby at Churchill Downs, Saturday, May 1, 2021, in Louisville, Ky. (AP Photo/Brynn Anderson) (Brynn Anderson/AP)